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A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity

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ACCOUNTS OF CHEMICAL RESEARCH
卷 37, 期 1, 页码 61-69

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AMER CHEMICAL SOC
DOI: 10.1021/ar030113y

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  1. NCI NIH HHS [CA41986, CA78045] Funding Source: Medline
  2. NATIONAL CANCER INSTITUTE [R01CA041986, P01CA078045] Funding Source: NIH RePORTER

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A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.

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