Precise quantitation of steroid 5 alpha lambda pi eta alpha-reductase type 1 mRNA levels by RT-PCR in female rat liver

The liver is a multifunctional organ capable of steroid hormone catabolism. The steroid metabolizing enzyme activities have been identified in the liver and it has been demonstrated that neonatal testicular androgens irreversibly program several hepatic enzyme activities in the rat including 5alpha-reductase (5alpha-R). In this paper we have applied a quantitative RT-PCR method coupled to laser-induced fluorescence capillary electrophoresis (LIF-CE) to measure the mRNA levels of 5alpha-R type 1 isozyme (5alpha-R1) in the liver of female intact and ovariectomized rats. Both groups were treated with testosterone (T) to study the possible regulation of 5alpha-R1 by androgens in females and to allow comparison with our previous findings in the liver of male rats. Our results demonstrate that the 5alpha-R1 mRNA isozyme is higher in the liver of female rats than in males and that the regulation of 5alpha-R1 mRNA by T is different in the liver of female than in male rats. Finally, our results could point to hepatic 5alpha-Reductase participation in the female not only in the catabolism of steroids with Delta(4,5) double bond, but also in other physiological functions such as the production of 3alpha,5alpha-reduced metabolites of progesterone and deoxycorticosterone (DOC), potent allosteric modulators of GABA(A) receptors.