期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 15, 期 8, 页码 971-980出版社
VSP BV
DOI: 10.1163/1568562041526469
关键词
sericin; sulfation; anticoagulant activity; gel-filtration chromatography
Silk sericin extracted from a cocoon was sulfated by chlorosulfonic acid. Sulfation of sericin was confirmed by FT-IR and the reaction efficiency was calculated as 44.9%. H-1-NMR suggested that sulfation mainly occurred at serine residues in the sericin molecule. The sulfated sericin was separated in three fractions by gel-filtration chromatography using Sephacryl S-200. The sulfate group content and amino-acid composition of each fraction were almost identical, while the anticoagulant activity differed for each fraction of sulfated sericin. Higher anticoagulant activity was ;observed for the higher-molecular-mass fraction. The anticoagulant activity of sulfated sericin was estimated at 1/10 to 1/20 of heparin.
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