期刊
HIPPOCAMPUS
卷 14, 期 5, 页码 557-569出版社
WILEY
DOI: 10.1002/hipo.10208
关键词
protein synthesis; hippocampus; CREB; NMDA; CaMKII; epinephrine
资金
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [F32NS010932] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG013622] Funding Source: NIH RePORTER
- NIA NIH HHS [R01 AG013622] Funding Source: Medline
- NINDS NIH HHS [F32 NS010932] Funding Source: Medline
Much attention has been paid to the associative processes that are necessary to fuse together representations of the various components of an episodic memory. In the present study, we focus on the processes involved in the formation of lasting representations of the individual components that make up a fear-conditioning episode. In one-trial contextual fear conditioning experiments, weak conditioning to context occurs if the shock is delivered immediately following placement of the animal in a novel conditioning apparatus, a phenomenon known as the immediate shock deficit. We show that the immediate shock deficit in mice may be alleviated by pre-exposure to either the context or shock. In using this approach to temporally dissect a contextual fear-conditioning task into its constituent representational and associative processes, we are able to examine directly the processes that are important for formation of lasting representations of the context conditioned stimulus (CS) or unconditioned stimulus (US). Our data indicate that the formation of a lasting representation of the context or shock engages protein synthesis-dependent processes. Furthermore, genetic disruption of cAMP-responsive element binding protein (CREB), a transcription factor that regulates the synthesis of new proteins required for long-term memory, disrupts the formation of lasting context memories. We go on to show that the stress hormone epinephrine modulates the consolidation of a context memory, and reverses consolidation deficits in the CREB-deficient mice. Finally we show that disrupting either NMDA or calcium/calmodulin-dependent kinase 11 (CaMKII) function impairs consolidation of context memories. Together, these data suggest that this approach is particularly suited for the characterization of molecular and cellular processes underlying the formation of stimulus representations. (C) 2004 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据