Polyamine, carbohydrate, and proline content changes during salt stress exposure of aspen (Populus tremula L.): Involvement of oxidation and osmoregulation metabolism

Excess salt in the soil solution affects the plant either through osmotic or ionic effects. Poplar trees, as fast growing pioneer tree species, are thought to be potential suitable candidates for afforestation on saline soils. Osmotic and oxidative stress induced by salinity could be reduced by the production and accumulation of compatible solutes and osmoprotectants in the plant. In this respect, metabolites of this type could be interesting markers for the improvement of salt stress tolerance in breeding programmes. Results have shown that Populus tremula was able to cope with up to 150 mM NaCl without any effect on plant survival. During stress application, the endogenous level of malondialdehyde did not vary significantly between the treatments, indicating that the level of lipid peroxidation was similar in the control and in the stressed plants. However, proline, spermine, sucrose, mannitol, and raffinose levels increased temporarily or throughout the salt treatment. All these molecules are more or less closely related to antioxidant or osmoprotectant mechanisms during stress, suggesting a key role for these compatible solutes, osmoprotectants, and their metabolism for salt stress resistance. The accumulation of free proline, sucrose and mannitol, and the transitory increase in spermine level observable during low and high NaCl application must be considered as general salt stress reaction markers.