期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 218, 期 5, 页码 731-739出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jeb.112664
关键词
Ion transporter; Osmoregulation; Prolactin; Rearing salinity; Tidal cycle; Tilapia
类别
资金
- National Science Foundation [IOS-1119693]
- Edwin W. Pauley Foundation
- Binational Agricultural Research Development (BARD) fund [IS-4296-10]
- National Oceanic and Atmospheric Administration [NA14OAR4170071]
- University of Hawaii Sea Grant College Program, SOEST [R/SS-12]
- University of Hawaii National Science Foundation EPSCoR program in support of the Core Genetics Facility at the Hawaii Institute of Marine Biology, University of Hawaii [EPS-0903833]
- University of Hawaii [UNIHI-SEAGRANT-JC-14-7]
This study characterizes the differences in osmoregulatory capacity among Mozambique tilapia, Oreochromis mossambicus, reared in freshwater (FW), in seawater (SW) or under tidally driven changes in salinity. This was addressed through the use of an abrupt exposure to a change in salinity. We measured changes in: (1) plasma osmolality and prolactin (PRL) levels; (2) pituitary expression of prolactin (PRL) and its receptors, PRLR1 and PRLR2; (3) branchial expression of PRLR1, PRLR2, Na+/Cl- co-transporter (NCC), Na+/K+/2Cl(-) co-transporter (NKCC), alpha 1a and alpha 1b isoforms of Na+/K+-ATPase (NKA), cystic fibrosis transmembrane conductance regulator (CFTR), aquaporin 3 (AQP3) and Na+/H+ exchanger 3 (NHE3). Mozambique tilapia reared in a tidal environment successfully adapted to SW while fish reared in FW did not survive a transfer to SW beyond the 6 h sampling. With the exception of CFTR, the change in the expression of ion pumps, transporters and channels was more gradual in fish transferred from tidally changing salinities to SW than in fish transferred from FW to SW. Upon transfer to SW, the increase in CFTR expression was more robust in tidal fish than in FW fish. Tidal and SW fish successfully adapted when transferred to FW. These results suggest that Mozambique tilapia reared in a tidally changing salinity, a condition that more closely represents their natural history, gain an adaptive advantage compared with fish reared in FW when facing a hyperosmotic challenge.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据