期刊
PHYTOCHEMICAL ANALYSIS
卷 15, 期 1, 页码 27-29出版社
WILEY
DOI: 10.1002/pca.738
关键词
HPLC-UV; spectrophotometric determination; colchicine; Jordanian medicinal plants; Colchicum steveni; Colchicum hierosolymitanum; Colchicaceae
The amounts of collchicine present in two Jordanian species of Colchicum, namely, C. steveni Knuth and C. hierosolyvmitanum Feibrun (Coichicaceae), have been determined. An HPLC-UV (photodiode array) method employing gradient elution was developed and the results compared with those obtained using a simple TLC-spectrophotometric method. The levels of colchicine as measured by these methods were not significantly different (p < 0.05) indicating that the spectrophotometric method is an acceptable alternative to HPLC. With respect to C steveni, the leaves contained the largest amount of collchicine (0.204/100 g), whilst in C. hierosolymitanum corms showed the highest collchicine content (0.126/100 g). As a source of colchicine, the two investigated species showed levels comparable with those found in C. autumnale, the traditional source of colchicine. Copyright (C) 2004 John Wiley Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据