Decolorization of synthetic dyes by immobilized spore from Bacillus amyloliquefaciens

Spore laccase from Bacillus amyloliquefaciens was evaluated for its dye decolorization capacity. More than 65% of the tested dyes were decolorized by the spore laccase in the presence of acetosyringone after 6 h. The spore laccase was entrapped in calcium alginate beads, and it could decolorize 99% of indigo carmine after fifteen cycles of repeated use. About 90% of simulated dye effluent was decolorized by the immobilized enzyme at pH 9.0 with 0.5 mM of acetosyringone as mediator. The results indicate a potential application of this immobilized spore laccase in treatment of textile dye effluents. (C) 2012 Elsevier B.V. All rights reserved.