The catalytic activity of cytochrome P450 3A22 is critical for the metabolism of T-2 toxin in porcine reservoirs

Contamination of food by T 2 toxin a trichothecene mycotoxin poses a serious threat to human health We report the identification of cytochrome P450 3A22 (CYP3A22) responsible for 3' hydroxylation of T-2 and HT-2 in pigs The present study shows that T-2 toxin significantly induces CYP3A22 expression in primary piglet hepatocytes Moreover recombinant pig CYP3A22 converted T-2 toxin into TC-1 (3'-OH T-2 toxin) and HT 2 toxin into TC-3 (3'-OH HT-2 toxin) in vitro These results suggest that pig CYP3A22 primarily eliminates T-2 and HT-2 toxins by 3'-hydroxylation of isovaleryl groups Therefore CYP3A22 is critical for xenobiotic metabolism and endogenous biochemical biotransformation of trichothecene mycotoxin in porcine reservoirs (C) 2010 Elsevier B V All rights reserved