期刊
JOURNAL OF NEUROIMMUNOLOGY
卷 146, 期 1-2, 页码 176-188出版社
ELSEVIER
DOI: 10.1016/j.jneuroim.2003.10.037
关键词
dendritic cell; interferon-beta; multiple sclerosis; monocyte
In addition to CD14++ classical monocytes, human peripheral blood contains CD14+CD16+ pro-inflammatory monocytes, which may be influenced by IFNb treatment. By fluorescence activated cell sorting (FACS) analysis, 94 multiple sclerosis (MS) patients revealed normal absolute and relative numbers of both monocyte populations (71 untreated, 23 IFNb-treated). In IFNb-treated patients, CD14+CD16+ monocytes consistently expressed higher CD14, confirmed in 16 patients analyzed longitudinally. Ex vivo, CD1a+CD14+ dendritic cells (DC) were efficiently differentiated from peripheral blood cells from controls and untreated patients, but at considerably reduced efficiency in IFNb-treated patients. Addition of IFNb to the medium further reduced the induction of CD1a+CD14+ cells. IFNb induces a novel immunophenotypic shift in pro-inflammatory monocytes, which appears to be related to reduced formation of dendritic cell precursors. (C) 2003 Elsevier B.V. All rights reserved.
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