期刊
NUCLEIC ACIDS RESEARCH
卷 32, 期 1, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gnh009
关键词
-
资金
- NCI NIH HHS [CA 43460, P50 CA062924, R37 CA057345, CA 62924, CA 57345, R01 CA057345, R37 CA043460] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [P50CA062924, R37CA043460, R37CA057345, R01CA043460, R01CA057345] Funding Source: NIH RePORTER
Emerging evidence suggests that recombinant adeno-associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an rAAV vector construction procedure employing fusion PCR and a single cloning step that considerably simplifies the knockout process. We demonstrate its utility by disrupting genes at specific positions within human colon cancer cells as well as within immortalized normal epithelial cells. This technology should be broadly applicable to in vitro studies that require the manipulation of the human genome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据