期刊
NEUROSCIENCE
卷 126, 期 3, 页码 665-676出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2004.04.008
关键词
neurotrophins; NGF; trkB; NMDA; AMPA/kainate; L-type voltage sensitive Ca2+ channels
Activity-dependent brain-derived neurotrophic factor (BDNF) expression is Ca2+-dependent, yet little is known about the Ca2+ channel contributions that might direct selective expression of the multiple BDNF transcripts. Here, effects of pilocarpine-induced seizure activity on total BDNF expression and on the individual sensitivity of BDNF transcripts to glutamate receptor and Ca2+ channel blockers were evaluated using hippocampal slice cultures and in situ hybridization of transcript-specific cRNA probes directed against mRNAs for the four 5' exons (I-IV) of the BDNF gene. mRNAs for nerve growth factor (NGF) and tyrosine kinase B (trkB) also were studied. Pilocarpine (5 mM) induced a dose- and time-dependent increase in total BDNF (exon V) mRNA expression in the dentate granule cells and CA3-CA1 pyramidal cells with maximal effects at 6 and 24 h, respectively. Increases were blocked by co-treatment with the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid/kainate 6-cyano7-nitroquinoxaline-2,3-dione (CNOX: 25 muM) and the N-methyl-D-aspartic acid receptor antagonist 2-amino-5-phosphonovaleric acid (APV; 25 muM), whereas the L-type voltage sensitive Ca2+ channel blocker nifedipine (20 muM) was without detectable effect. Maximal NGF and trkB mRNA expression was induced by pilocarpine at 4 and 12 h, respectively. For the individual BDNF transcripts, APV blocked pilocarpine-induced increases in transcript 11, whereas nifedipine blocked increases in transcripts I and Ill. Transcript IV levels were not altered by treatment. These results indicate that transcript 11 makes the greatest contribution to pilocarpine effects on total BDNF mRNA content in this model and provides evidence for regional and Ca2+ channel-specific differences in activity-dependent regulation of the different BDNF transcripts in hippocampus. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.
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