期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 286, 期 1, 页码 H68-H75出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00245.2003
关键词
calcium handling; gene therapy
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL066917] Funding Source: NIH RePORTER
- NHLBI NIH HHS [HL-52496, HL-66917] Funding Source: Medline
In many types of heart failure cardiac myocyte Ca2+ handling is abnormal because of downregulation of key Ca2+ - handling proteins like sarco( endo) plasmic reticulum Ca2+- ATPase ( SERCA) 2a and ryanodine receptor ( RyR) 2. The alteration in SERCA2a and RyR2 expression results in altered cytosolic Ca2+ transients, leading to abnormal contraction. Sorcin is an EF- hand protein that confers the property of caffeine- activated intracellular Ca2+ release in nonmuscle cells by interacting with RyR2. To determine whether sorcin could improve the contractile function of the heart, we overexpressed sorcin in the heart of either normal or diabetic mice and in adult rat cardiomyocytes with an adenoviral gene transfer approach. Sorcin overexpression was associated with an increase in cardiac contractility of the normal heart and dramatically rescued the abnormal contractile function of the diabetic heart. These effects could be attributed to an improvement of the Ca2+ transients found in the cardiomyocyte after sorcin overexpression. Viral vector- mediated delivery of sorcin to cardiac myocytes is beneficial, resulting in improved contractile function in diabetic cardiomyopathy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据