期刊
DEVELOPMENT
卷 131, 期 2, 页码 435-446出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.00922
关键词
dauer; cyclin dependent kinase inhibitor; dachshund box; nematode
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM060994] Funding Source: NIH RePORTER
- NIGMS NIH HHS [GM60994] Funding Source: Medline
An unconventional TGFbeta superfamily pathway plays a crucial role in the decision between dauer diapause and reproductive growth. We have studied the daf-5 gene, which, along with the daf-3 Smad gene, is antagonized by upstream receptors and receptor-regulated Smads. We show that DAF-5 is a novel member of the Sno/Ski superfamily that binds to DAF-3 Smad, suggesting that DAF-5, like Sno/Ski, is a regulator of transcription in a TGFbeta superfamily signaling pathway. However, we present evidence that DAF-5 is an unconventional Sno/Ski protein, because DAF-5 acts as a co-factor, rather than an antagonist, of a Smad protein. We show that expressing DAF-5 in the nervous system rescues a daf-5 mutant, whereas muscle or hypodermal expression does not. Previous work suggested that DAF-5 and DAF-3 function in pharyngeal muscle to regulate gene expression, but our analysis of regulation of a pharynx specific promoter suggests otherwise. We present a model in which DAF-5 and DAF-3 control the production or release of a hormone from the nervous system by either regulating the expression of biosynthetic genes or by altering the connectivity or the differentiated state of neurons.
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