期刊
ANALYTICAL BIOCHEMISTRY
卷 324, 期 1, 页码 79-83出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2003.09.008
关键词
bismuth citrate; inorganic phosphate; Na/K ATPase; alkaline phosphatase
This paper describes a rapid and sensitive method to determine inorganic phosphate, even in the presence of labile organic phosphate compounds and large quantities of proteins. The method eliminates the use of sodium arsenite, a highly toxic compound, substituting bismuth citrate for it to stabilize the phosphomolybdic acid complex formed during the interaction of inorganic phosphate and molybdate reduced by ascorbic acid. This method has also been adapted to microplates and has been used to determine the activities of Na/K ATPase and alkaline phosphatase of intestinal basolateral and luminal plasma membranes. (C) 2003 Elsevier Inc. All rights reserved.
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