We report a new approach to greatly simplify the fabrication of nanofluidic channels with well-controlled dimensions. It is achieved by imprinting a channel template into a thin polymer film cast on a glass cover slip in a single step, offering a much higher throughput than previous methods. We demonstrated effective DNA stretching in these nanochannels. This method provides a simple and practical solution for low-cost fabrication of nanofluidic channels, which may serve as a useful tool for chemical analysis system on the nanoscale.
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