期刊
ELECTROPHORESIS
卷 25, 期 2, 页码 214-228出版社
WILEY
DOI: 10.1002/elps.200305745
关键词
band broadening; capillary electrophoresis; electroosmosis; lubrication theory; review; Taylor dispersion
Electroosmotic flow (EOF) usually accompanies electrophoretic migration of charged species in capillary electrophoresis unless special precautions are taken to supress it. The presence of the EOF provides certain advantages in separations. It is an alternative to mechanical pumps, which are inefficient and difficult to build at small scales, for transporting reagents and analytes on microfluidic chips. The downside is that any imperfection that distorts the EOF profile reduces the separation efficiency. In this paper, the basic facts about EOF are reviewed from the perspective of fluid mechanics and its effect on separations in free solution capillary zone electrophoresis is discussed in the light of recent advances.
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