期刊
JOURNAL OF MATERIALS CHEMISTRY
卷 14, 期 14, 页码 2311-2316出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b401902d
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Anti-cortisol was encapsulated in optically transparent sol - gel silica matrices and competitive immunoassays for cortisol were conducted using the antibody-doped silica material as sensing elements. Calibration curves were derived in the physiological relevant range for cortisol of 1 to 100 mug dl(-1). Sensing elements were prepared in both monolith and thin-film forms, and results show that with thin films, accessibility of antigen to the encapsulated antibody was improved leading to a significant reduction in the required assay time. Moreover, non-specific binding to the sol - gel silica matrix was well controlled.
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