期刊
CARDIOVASCULAR RESEARCH
卷 80, 期 2, 页码 227-235出版社
OXFORD UNIV PRESS
DOI: 10.1093/cvr/cvn192
关键词
Ginkgo; Doxorubicin; Apoptosis; p53; Cardiomyocyte
资金
- Yen-Tjing-Ling Medical Foundation [CI-96]
- Taichung Veterans General Hospital [TCVGH-963108C, TCVGH-966303C]
Aims The objective of this study was to investigate whether a cytoprotective herb-derived agent, Ginkgo biloba extract (EGb) 761, could have a beneficial effect on doxorubicin-induced cardiac toxicity in vitro and in vivo. Methods and results Primary cultured neonatal rat cardiomyocytes were treated with the vehicle, doxorubicin (1 mu M), EGb761 (25 mu g/mL), or EGb761 plus doxorubicin. After 24 h, doxorubicin upregulated p53 mRNA expression, disturbed Bcl-2 family protein balance, disrupted mitochondrial membrane potential, precipitated mitochondrion-dependent apoptotic signalling, induced apoptotic cell death, and reduced viability of cardiomyocytes, whereas EGb761 pretreatment suppressed all the actions of doxorubicin. Similarly, rats treated with doxorubicin [3 mg/kg intraperitoneally (i.p.) three doses every other day] displayed retarded growth of body and heart as well as elevated apoptotic indexes in heart tissue at both 7 and 28 days after exposure, whereas EGb761 pretreatment (5 mg/kg i.p. 1 day before each dose of doxorubicin) effectively neutralized the aforementioned gross and cellular adverse effects of doxorubicin. Conclusion Doxorubicin impairs viability of cardiomyocytes at least partially by activating the p53-mediated, mitochondrion-dependent apoptotic signalling. EGb761 can effectively and extensively counteract this action of doxorubicin, and may potentially protect the heart from the severe toxicity of doxorubicin.
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