期刊
CARDIOVASCULAR PATHOLOGY
卷 17, 期 1, 页码 6-13出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.carpath.2007.01.005
关键词
MRL; cardiac regeneration; myocardial infarction; cryoinjury; scar
资金
- NHLBI NIH HHS [R01 HL061553, P01 HL003174-52, R24 HL64387, P01 HL03174, R01 HL61553, R24 HL064387, P01 HL003174] Funding Source: Medline
- NIBIB NIH HHS [T32 EB001650] Funding Source: Medline
- NIGMS NIH HHS [P01 GM081619, T32 GM007266, P01 GM081619-010003, 5T32 GM07266] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R24HL064387, P01HL003174, R01HL061553] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [T32EB001650] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM007266, P01GM081619] Funding Source: NIH RePORTER
Background: Myocardial infarcts in mammals heal by scar formation rather than formation of new muscle tissue. The MRL/MpJ [Murphy Roths large (MRL) derived by the Murphy group of the Jackson Laboratory (MpJ)] mouse, however, has been reported to exhibit minimal scarring and subsequent cardiac regeneration after cryoinjury of the right ventricle. Other groups have reported that permanent and temporary ligation of the coronary artery resulted in scarring without regeneration. Methods: To clarify these contradictory results, we studied the temporal evolution of infarcts in MRL/MpJ and C57BL/6 control mice from I to 90 days post injury and the effects of intrathoracic cryoinjury to 28 days. Results: After infarction, the conversion from necrotic myocardium to granulation tissue and then to scar proceeded identically in the two groups. Infarct DNA synthesis, measured by incorporation of a 5-bromo-2-deoxyuridine pulse, peaked at 4 days in both strains and did not differ between strains at any time point. Endothelial cell and total vascular density in the both the infarcted and noninfarcted cardiac tissue did not differ between groups at any time. Histological analysis of directly cryoinjured right and left ventricular myocardium showed indistinguishable wound healing in both strains, and final scar size was identical in each group. Conclusions: These studies demonstrate that both myocardial infarcts and cryoinjuries in MRL/MpJ mice heal by typical scar formation rather than muscle regeneration, in a manner very similar to C57BL/6 controls. We conclude that the MRL mouse is not a model for myocardial regeneration. (c) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据