期刊
PLANT SCIENCE
卷 166, 期 1, 页码 69-79出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2003.08.008
关键词
cold-responsive genes; T-DNA; rice; demethylmenaquinone methyltransferase
To identify low-temperature-responsive genes in rice, we screened gus-trapped T-DNA tagging lines that had been subjected to cold stress at 5 degreesC. Out of 15,586 lines, 81 (0.52%) showed cold-responsive GUS activity. Among the 62 lines we selected for further study, 53 showed increased GUS activity while 9 showed less. In addition, 16 of those 62 lines were influenced by abscisic acid (ABA), suggesting an ABA-dependent cold response. We used inverse PCR or thermal asymmetric interlaced (TAIL) PCR to identify 37 tagged genes from those lines, and further characterized two of them at the molecular level. The OsRLK1 gene, which encodes a putative leucine rich repeat (LRR)-type receptor-like protein kinase, was inducible by cold and salt stresses. The OsDMKT1 gene, encoding a putative demethylmenaquinone methyltransferase (DMKT), was also inducible by low temperatures, but not by high salt or drought. The T-DNA-tagged OsDMKT1 gene also co-segregated with the cold-inducible gus gene. Our results indicate that the T-DNA tagging lines are useful in obtaining the stress-responsive genes in rice. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据