Decolorization of reactive azo dyes by Cunninghamella elegans UCP 542 under co-metabolic conditions

The inappropriate disposal of dyes in wastewater constitutes an environmental problem and can cause damage to the ecosystem. Alternative treatments have been reported that fungi are particularly effective in the decolorization of textile effluents. The decolorization of dyes with different molecular structures by Cunninghamella elegans was evaluated under several media conditions. The decolorization procedures consisted of adding 72 h of mycelium into the culture medium containing either orange or reactive black or reactive red or a mixture of these dyes in the presence or absence of sucrose and/or peptone. The decolorization profile was highly dependent upon the incubation time, the molecular structure of the dye and presence or absence of co-substrates. The presence of sucrose or both sucrose and peptone significantly increased the decolorization of the solutions, however, the presence of only the nitrogen source suppressed it. The ultraviolet spectra of the solutions before and after decolorization suggested the occurrence of biodegradation in addition to the biosorption of the dyes. All tested dyes, except for the reactive black, caused inhibition of respiration of Escherichia coli, which suggested that toxic metabolites were produced. (C) 2003 Elsevier Ltd. All rights reserved.