期刊
JOURNAL OF OPTICS A-PURE AND APPLIED OPTICS
卷 6, 期 1, 页码 89-93出版社
IOP PUBLISHING LTD
DOI: 10.1088/1464-4258/6/1/016
关键词
optical tweezers; optical scalpel; chromosome; rice genome
类别
A new method based on optical tweezers technology is reported for the isolation of a single chromosome. A rice cell suspended in liquid was first fragmented by laser pulses (optical scalpel). Then a single released chromosome from the cell was manipulated and pulled away from other cells and oddments by optical tweezers without any direct mechanical contact. Finally the isolated single chromosome was extracted individually into a glass capillary nearby. After molecular cloning of the isolated chromosome, we obtained some specific DNA segments from the single chromosome. All these segments can be used for rice genomic sequencing. Different methods of extracting a single chromosome are compared. The advantages of optical micromanipulation method are summarized.
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