期刊
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
卷 23, 期 10, 页码 1657-1666出版社
TAYLOR & FRANCIS INC
DOI: 10.1081/NCN-200031467
关键词
DNA; conjugation; oxime; peptide; thiazolidine
Oligonucleotides carrying an aldehyde moiety at the 3'-end were synthesized by the oxidation of a 1,2-diol precursor. These were coupled to peptides bearing a cysteine residue for thiazolidine formation and an aminooxy group for oxime formation. The conjugation reaction proved very efficient and selective, thereby allowing the preparation of 3'-peptide-oligonucleotide conjugates in good yield. The conjugation was achieved in aqueous solution without using any protection strategy. Moreover, the present approach neither requires the use of peptide in excess nor changes the hybridization properties of the conjugates.
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