期刊
TISSUE ENGINEERING
卷 11, 期 9-10, 页码 1449-1458出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.2005.11.1449
关键词
-
资金
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [Z01DE000336, ZIADE000336] Funding Source: NIH RePORTER
To reengineer polarized epithelial cell functions directly in situ, or ex vivo in the fabrication of an artificial organ, it is necessary to understand mechanisms that account for polarized membrane sorting. We have used the aquaporins ( AQPs), a family of homotetrameric water channel proteins, as model membrane proteins for this purpose. AQP monomers contain six transmembrane-spanning domains linked by five interconnecting loops, with the NH2 and COOH termini residing in the cytosol. AQP5 is localized in the apical membranes of several different epithelia in vivo, and in stably transfected MDCK-II cells grown as a polarized monolayer. We wished to identify a structural region( s) within rat AQP5 ( rAQP5) important for apical localization, and to study the MDCK-II cell localization of rAQP5s modified in either their NH2 or COOH terminus. We show that the NH2-terminal region does not play a major role in apical localization as deletion of the NH2 terminus produced a modified rAQP5 construct ( AQP5-NTdel) that was stably expressed and localized primarily to the apical membranes of MDCK-II cells. Attachment of a FLAG epitope to the NH2 terminus of AQP5 ( AQP5(flag) construct) also did not perturb apical localization. In addition, we found that the exchange of NH2-terminal regions between rAQP5 and human AQP1 ( hAQP1; a nonpolarized AQP isoform) produced a modified rAQP5 construct ( AQP5-1NT) and a modified hAQP1 construct ( AQP1-5NT), each of which localized as the parental AQP ( apically, and to both apical and basolateral membranes, respectively). In contrast, we found that deletion of the COOH terminus resulted in a modified rAQP5 construct ( AQP5-CTdel) that was unstably expressed and localized to intracellular site( s) in MDCK-II cells. Substitution of the COOH terminus of AQP1 with the COOH terminus of AQP5 also produced a construct ( AQP1-5CT) transiently expressed in intracellular compartment( s). However, substitution of the COOH terminus of rAQP5 with the COOH terminus of hAQP1 produced a modified rAQP5 construct ( AQP5-1CT) that was stably expressed and localized to basolateral membranes, suggesting the loss of an apical targeting/ retention signal from rAQP5, the gain of a basolateral targeting/ retention signal from hAQP1, or a combination of these two possibilities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据