期刊
JOURNAL OF ENDOCRINOLOGY
卷 184, 期 1, 页码 153-163出版社
BIOSCIENTIFICA LTD
DOI: 10.1677/joe.1.05935
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资金
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [F32HD008693] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [R37MH041788] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P01AG018911] Funding Source: NIH RePORTER
- NIA NIH HHS [P01 AG018911] Funding Source: Medline
- NICHD NIH HHS [F32 HD08693, F32 HD008693] Funding Source: Medline
- NIMH NIH HHS [R37 MH41788] Funding Source: Medline
The circadian glucocorticoid rhythm provides important information on the functioning of the hypothalamic-pituitary-adrenal axis in individuals. Frequent repeated blood sampling can limit the kinds of studies conducted on this rhythm, particularly in small laboratory rodents that have limited blood volumes and are easily stressed by handling. We developed an extraction and assay protocol to measure fecal corticosterone metabolites in repeated samples collected from undisturbed male and female adult Sprague-Dawley rats. This fecal measure provides a non-invasive method to assess changes in corticosterone within a single animal over time, with sufficient temporal acuity to quantify several characteristics of the circadian rhythm: e.g. the nadir, acrophase, and asymmetry (saw-tooth) of the rhythm. Males excreted more immunoreactive fecal corticoids than did females. Across the estrous cycle, females produced more fecal corticoids on proestrus (the day of the preovulatory luteinizing hormone surge) than during estrus or metestrus. These results establish a baseline from which to study environmental, psychological, and physiological disturbances of the circadian corticosterone rhythm within individual rats.
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