期刊
JOURNAL OF BIOCHEMISTRY
卷 137, 期 1, 页码 33-39出版社
JAPANESE BIOCHEMICAL SOC
DOI: 10.1093/jb/mvi007
关键词
catalytic domain; expression cloning; immunoscreening; Lotus japonicus; protein kinase
To investigate protein kinases expressed in Lotus japonicus, a cDNA expression library of the root-nodule of L. japonicus was immunologically screened with monoclonal antibodies directed to a highly conserved region in protein serine/threonine kinases (Ser/Thr kinases). Among 178 positive clones obtained from the lambda ZAPII cDNA library, 164 clones were found to encode novel proteins possessing the subdomain VIE sequences characteristic of Ser/Thr kinases. By phylogenetic analysis on the basis of deduced amino acid sequences, the isolated clones could be classified into five different families of Ser/Thr kinases : the SnRK family, GSK-3 family, Ndr kinase family, Ark family, and receptor kinase family. These results suggest that this expression cloning using the kinase-specific antibodies will provide new clues for investigations of a wide variety of known and novel protein kinases in higher plants.
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