期刊
INFLAMMATORY BOWEL DISEASES
卷 11, 期 1, 页码 16-23出版社
WILEY-BLACKWELL
DOI: 10.1097/00054725-200501000-00003
关键词
Crohn's disease; interleukin-23; interleukin-27; real-time polymerase chain reaction; ulcerative colitis
Background: It has been suggested that Crohn's disease (CD) is associated with an exaggerated T-helper 1 cytokine response manifested by increased production of interleukin (IL)-12. IL-12 is a heterodimeric protein comprising 2 disulfide-linked subunits designated p35 and p40. Recently, IL-12-related cytokines, IL-23 and IL-27, were described. Biologically active IL-23 is a heterodimer whose p40 subunit is identical to IL-12p40 whereas its p19 subunit is distantly related to IL-12p35. IL-27 consists of EB13, an IL-12p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide. Aim: We Sought to determine whether mucosal expression of IL-23p 19 and IL-27p28 transcripts correlate with the inflammatory activity in inflammatory bowel disease (IBD). Patients/Methods: Messenger RNA expression in colonic mucosa from patients with Crohn's disease (CD; n = 37) and ulcerative colitis (UC; n = 19), and in non-IBD control subjects (specific colitis [SC]; n = 16) and normal, nondiseased control patients (n = 12) was measured by reverse-transcribed real-time polymerase chain reaction. Results: IL-23p19 was significantly increased in inflamed mucosa in CD (P = 0.0377) and to a lesser extent also in UC patients but not in SC patients. Elevation of IL-23p 19 transcript levels in CD correlated with the severity of endoscopic lesions. IL-27p28 transcripts and EB13 transcripts were significantly elevated only in active CD. Discussion: IL-23p 19, IL-27p28, and EB13 transcripts are strongly Up-regulated in CD. The stimulatory effects of these cytokines on naive T cells in addition to a strongly synergistic action with IL-12 to trigger interferon-gamma production may contribute to the perpetuation of the inflammatory process in patients with CD. Notably, increased expression of IL-23 and IL-27 transcripts in CD suggests a T helper 1-dominated immunologic function in this disease.
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