The characteristic and application of molecularly imprinted polymer: Efficient sample preconcentration of antibiotic cefathiamidine from human plasma and serum by solid phase extraction

Polymers imprinted with cefathiamidine were synthesized using 4-vinyl-pyridine as an interacting monomer, ethyleneglycol dimethacrylate as a cross-linker, and methanol and toluene as a porogen. The polymer was used as solid-phase extraction sorbent. One-hundred percent of the cefathiamidine was bound to the polymer and the target can be eluted efficiently only by 1 ml eluant. Human plasma and serum samples were preconcentrated by the molecularly imprinted solid-phase extraction prior to analysis by HPLC. Recoveries of up to 98% were achieved. The molecularly imprinted solid phase extraction column can be used repeatedly.