期刊
JOURNAL OF ORTHOPAEDIC RESEARCH
卷 23, 期 1, 页码 102-108出版社
WILEY
DOI: 10.1016/j.orthres.2004.06.015
关键词
MRI; T-1p; cartilage; IL-1; osteoarthritis; proteoglycan
类别
资金
- NCRR NIH HHS [RR02305, P41 RR002305-236879, P41 RR002305] Funding Source: Medline
- NIAMS NIH HHS [R01-AR45404, R01 AR045404] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR002305] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR045404] Funding Source: NIH RePORTER
The purpose of this work is to demonstrate the feasibility of T-1p-weighted magnetic resonance imaging (MRI) to quantitatively measure changes in proteoglycan content in cartilage. The T-1p MRI technique was implemented in an in vivo porcine animal model with rapidly induced cytokine-mediated cartilage degeneration. Six pigs were given an intra-articular injection of recombinant porcine interleukin-1beta (IL-1beta) into the knee joint before imaging to induce changes in cartilage via matrix metalloproteinase (MMP) induction. The induction of MMPs by IL-1 was used since it has been extensively studied in many systems and is known to create conditions that mimic in part characteristics similar to those of osteoarthritis. The contralateral knee joint was given a saline injection to serve as an internal control. T-1p-weighted MRI was performed on a 4 T whole-body clinical scanner employing a 2D fast spin-echo-based T-1p imaging sequence. T-1p relaxation parameter maps were computed from the T-1p-weighted image series. The average T-1p relaxation rate, R-1p (l/T-1p) of the IL-1beta-treated patellae was measured to be on average 25% lower than that of saline-injected patellae indicating a loss of proteoglycan. There was an average reduction of 491X, in fixed charge density, measured via sodium MRI of the IL-1beta-treated patellae relative to control corroborating the loss of proteoglycan. The effects of IL-1beta primarily loss of PG, were confirmed by histological and immunochemical findings. The results from this study demonstrate that R-1p is able to track proteoglycan content in vivo. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据