期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 162, 期 -, 页码 155-162出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2014.12.049
关键词
Kai-Xin-San; Optimization; Paired-herb; Neurofilament; Neuronal differentiation
资金
- Hong Kong Research Grants Council Theme-based Research Scheme [T13-607/12R]
- GRF [661110, 662911, 660411, 663012, 662713]
- Hong Kong jockey Club Charities Trust
- Foundation of The Awareness of Nature [TAON12SC01]
- Kanya Lee Scholarship from Lee's Pharmaceutical C. Ltd. [10839916]
- [TUYF12SC03]
Ethnopharmacological relevance: Kai-Xin-San (KXS), a well-known traditional Chinese herbal decoction, has been widely used to treat mental depression and memory loss in China. It has a combination of four herbs: Ginseng Radix et Rhizoma (GR; root and rhizome of Panax ginseng C.A. Mey.), Polygalae Radix (PR; root of Polygala tenuifolia Wild.), Acori Tatarinowii Rhizoma (ATR; rhizome of Acorus tatarinowii Schott), and Poria (PO; sclerotium of Poriacocos (Schw.) Wolf), from which a pairing of two herbs was considered as paired-herb, such as the pairing of GR-PR and ATR-PO. The depression-induced neural cell loss is one of the major pathogenesis in depression. Here, an optimized ICXS by changing the ratio of paired-herbs in KXS was demonstrated aiming at promoting neural cell differentiation. Materials and methods: Quantitative assessment of chemical markers in each herbal extract was determined by LC-MS. Promoters of neurofilaments, NF68 and NF200, linked with luciferase reporter gene (pNF68-Luc and pNF200-Luc) were applied in cultured pheochromocytoma (PC12) cells to study the transcriptional activation of each herbal extract. The effect of GR-PR and ATR-PO in improving NF promoter activity was analyzed by Compusyn software. The activation of PICA was indicated. Results: In PC12 cells, an optimized KXS named KXS1:5 having 1:5 of GR-PR:ATR-PO had greater capability in promoting the expression of neurofilament. The synergistic effect of GR-PR and ATR-PO on the improved efficiency was further determined. Moreover, the treatment of H89, a PICA inhibitor, significantly inhibited the induced NF promoter activity. Conclusion: These results indicated an optimized 106 by optimizing the compatibility of paired-herb and this compatibility was proven to exert synergistic effect. Moreover, the underlying mechanism was mediated by a PICA signaling pathway. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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