期刊
PEST MANAGEMENT SCIENCE
卷 61, 期 1, 页码 91-96出版社
WILEY
DOI: 10.1002/ps.954
关键词
Myzus persicae; insecticide resistance; MACE; kdr
The peach-potato aphid Myzus persicae (Sulzer) has developed a number of insecticide resistance mechanisms owing to the high selective pressure produced by world-wide insecticide treatments. Knowledge of the geographical distribution and the temporal evolution of these resistant phenotypes helps to develop suitable pest-management programs. Current understanding of the major mechanisms of resistance at the molecular level makes it possible to diagnose the presence of modified acetylcholinesterase (MACE) or knockdown resistance (kdr). This paper describes a rapid method for the identification of both resistance mechanisms in a single molecular assay by using restriction fragment length polymorphism of PCR products (RFLP-PCR) in individual as well as pooled aphids. (C) 2004 Society of Chemical Industry.
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