期刊
JOURNAL OF NEURAL TRANSMISSION
卷 112, 期 1, 页码 77-85出版社
SPRINGER WIEN
DOI: 10.1007/s00702-004-0171-6
关键词
intracellular sodium; energy metabolism; fluorescence microscopy
Glutamate uptake into astrocytes and the resulting increase in intracellular Na+ (Na-i(+)) have been identified as a key signal coupling excitatory neuronal activity to increased glucose utilization. Arguments based mostly on mathematical modeling led to the conclusion that physiological concentrations of glutamate more than double astrocytic Na+/K+-ATPase activity, which should proportionally increase its ATP hydrolysis rate. This hypothesis was tested in the present study by fluorescence monitoring of free Mg2+ (Mg-i(2+)), a parameter that inversely correlates with ATP levels. Glutamate application measurably increased Mg-i(2+) (i.e. decreased ATP), which was reversible after glutamate washout. Na-i(+) and ATP changes were then directly compared by simultaneous Na-i(+) and Mg2+ imaging. Glutamate increased both parameters with different rates and blocking the Na+/K+-ATPase during the glutamate-evoked Na-i(+) response, resulted in a drop of Mg-i(2+) levels (i.e. increased ATP). Taken together, this study demonstrates the tight correlation between glutamate transport, Na+ homeostasis and ATP levels in astrocytes.
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