期刊
PHARMACOLOGY
卷 73, 期 2, 页码 97-101出版社
KARGER
DOI: 10.1159/000081625
关键词
nerve growth factor; dorsal root ganglion; vanilloid receptor; 3 beta-hydroxysteroid dehydrogenase; neuroregeneration
It was the aim of the present study to investigate by RTPCR the regulation of the mRNA of the neurosteroid-synthesizing enzyme 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and of the vanilloid receptor TRPV1 in dorsal root ganglia ( DRGs) of rats during the process of capsaicin denervation of primary sensory neurons and the following regeneration. The expression of 3beta-HSD in DRG was increased 3 days after the capsaicin treatment, and it remained at that level during a 22 day observation period. The expression of TRPV1, a specific marker of capsaicin-sensitive small sensory neurons connected to C- and Adelta-fibers, was markedly reduced 3 days after the capsaicin treatment. It slowly recovered during the 22 days observation period reaching almost control levels on day 22. When the capsaicin-treated rats received 5 intraplantar injections of nerve growth factor (NGF), the prototypical neurotrophin for capsaicin-sensitive neurons, on day 1, 2, 3, 5 and 6, both the 3beta-HSD and the TRPV1 mRNA had returned to control levels at the time point 8 days after capsaicin. The present results demonstrate that both 3beta-HSD and TRPV1 are markers for neurodegeneration and neuroregeneration in capsaicin-sensitive primary afferent neurons, and that NGF is an effective tool to induce recovery after peripheral nerve injury. Copyright (C) 2005 S. Karger AG, Basel.
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