期刊
BIOMEDICAL CHROMATOGRAPHY
卷 19, 期 7, 页码 518-522出版社
WILEY
DOI: 10.1002/bmc.473
关键词
tricin; HPLC; mouse; plasma; liver; intestinal mucosa
AHPLC method developed and validated for the determination of tricin in human plasma published previously was cross-validated to allow measurement of the flavone tricin in plasma and tissues of mice. Blank samples of plasma, liver or intestinal mucosa were spiked with tricin at 0.5-4.0, 1.0-8.0 and 5.0-40 mu g/mL, respectively. These tricin concentration ranges covered the tricin levels achieved in the mouse tissues in the dose-escalating experiments. Analysis afforded linear calibration curves with regression coefficients of > 0.99. Endogenous compounds did not interfere with tricin detection when the detection wavelength was set at 355 nm, the maximum absorbance of tricin. Accuracy and precision were < 15% for all concentrations in all matrices except for the precision at the lower limit of quantification (0.5 mu g/mL) in mouse plasma, which was 18.4%. Consumption of diet mixed with tricin at 0.05, 0.2 or 0.5% for one week furnished steady-state levels in plasma, liver and small intestine in the 1-3 x 10(-7), 4-22 x 10(-7) and 3-46 x 10(-5) M ranges, respectively. Copyright (c) 2005 John Wiley & Sons, Ltd.
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