Cellular localization and photodynamic effect of chlorin e6-monoclonal antibody conjugate

Chlorin e6 (Ce6) was conjugated with anti-tumor monoclonal antibody (IgG) to increase its binding affinity for tumors. Ce6 was activated by N-hydroxysulfosuccinimide and conjugated with IgG via peptide bonds, and Ce6 molecules were conjugated with IgG (IgG-Ce6) and their binding affinities to tumor cells were investigated. Intracellular localization of IgG-Ce6 was observed, and IgG-Ce6 was accumulated in tumor cells much higher than Ce6, indicating that the IgG-Ce6 has specific binding affinity to tumor cells. The effective photocytotoxicity of the cells with IgG-Ce6 is caused by the high accumulation of IgG-Ce6 in tumor cells. Copyright (c) 2005 Society of Porphyrins & Phthalocyanines.