期刊
CLINICAL & EXPERIMENTAL METASTASIS
卷 22, 期 1, 页码 47-58出版社
SPRINGER
DOI: 10.1007/s10585-005-2908-5
关键词
Akt; breast; breast carcinoma; cell line metastasis; comparative genomic hybridization; ERBB2; gene expression analysis; LY294002; mammary fat pad; mouse mammary tumor virus long terminal repeat; oncogene; orthotopic; phosphatidylinositol 3 kinase; polyoma middle T; pulmonary metastasis; Sept9; Spp1 osteopontin; Opn; syngeneic; transgenic; tumor transplant
类别
资金
- NATIONAL CANCER INSTITUTE [P30CA093373, Z01CP010146] Funding Source: NIH RePORTER
- NCI NIH HHS [P30 CA 93373-01, R01 CA 81736] Funding Source: Medline
Two cell lines, Met-1(fvb2) and DB-7(fvb2), with different metastatic potential, were derived from mammary carcinomas in FVB/N-Tg(MMTV-PyVmT) and FVB/N-Tg(MMTV-PyVmT(Y315F/Y322F)) mice, transplanted into syngeneic FVB/N hosts and characterized. The lines maintain a stable morphological and biological phenotype after multiple rounds of in vitro culture and in vivo transplantation. The Met-1(fvb2) line derived from a FVB/N-Tg(MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. The DB-7(fvb2) line derived from the FVB/N-Tg(MMTV-PyVmT(Y315F/Y322F)) with a double base modification at Y315F/Y322F exhibits more rapid growth when transplanted into the mammary fat pad, but a lower rate of metastasis (17%). The Met1(fvb2) cells show high activation of AKT, while DB-7(fvb2) cells show very low levels of AKT activation. The DNA content and gene expression levels of both cell lines are stable over multiple generations. Therefore, these two cell lines provide a stable, reproducible resource for the study of metastasis modulators, AKT molecular pathway interactions, and gene target and marker discovery.
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