期刊
CANCER DETECTION AND PREVENTION
卷 29, 期 4, 页码 323-331出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cdp.2005.06.010
关键词
estrogen receptor; myoepithelial cells; anti-mitotic agents; stromal and vascular invasion; stem cells; breast tumor cells; ductal carcinoma in situ; morphologic features; progression-related markers; tumor invasion and metastasis; vascular invasion; immunostataining; matrix metalloproteinase-26; c-erb-B2 protein; cytokeratin; basement membrane disruption; genetic alterations
类别
资金
- NCI NIH HHS [CA91149, CA78646, CA65532] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R21CA091149, R29CA078646, R01CA065532] Funding Source: NIH RePORTER
Background: Our previous studies in pre-invasive mammary tumors revealed that estrogen receptor negative cell clusters (ER NCC) overlying focally disrupted myoepithelial (ME) cell layers showed a significantly higher rate of genetic abnormalities and cell proliferation than adjacent cells without ME cell layer disruptions. A subset of these ER NCC, however, completely lacked expression of Ki-67, a most commonly used marker for cell proliferation. The purpose of this study was to further elucidate the immunohistochemical and morphological profiles of these ER NCC. Methods: Fifteen cases with such ER NCC were selected from our previous studies and assessed with a panel of commonly used biomarkers for cell proliferation, tumor progression, and normal stem cells. Results: Immunohistochemically, in addition to Ki-67 and ER. these ER NCC completely lacked expression of all other proliferation and progression related markers that were distinctly expressed in adjacent cells within the same duct but overlying the non-disrupted ME cell layer. These ER NCC also lacked expression of all normal stem cell-related markers tested. These cell clusters, however, showed a higher and atypical expression of c-erb-B2, compared to their adjacent counterparts. Morphologically, these ER NCC were generally arranged as triangle shaped structures penetrating into the stroma, similar to micro-invasive lesions. About 15% of these ER NCC appeared to directly spread into blood vessel-like structures. These ER NCC and their possible derivatives within the stroma and blood vessels-like structures shared the same morphologic and immunohistochemical features. No comparable ER positive cell clusters were identified in any of the cases. Conclusions: These findings suggest that these ER NCC and their possible derivatives are likely regulated by yet to be defined molecules and mechanisms, and they are unlikely to respond to currently available anti-mitotic agents. (c) 2005 International Society for Preventive Oncology. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据