期刊
ACTA HISTOCHEMICA
卷 107, 期 2, 页码 143-148出版社
URBAN & FISCHER VERLAG
DOI: 10.1016/j.acthis.2005.01.003
关键词
immunocytochemistry; immunohistology; multiple immuno-labeling; monoclonal primary antibodies
类别
immunolabeling method with primary antibodies originating from the same host species. Here, we briefly outline different approaches intended to close this technological gap and focus on multiple immunolabeling with monoclonal primary antibodies. To this end, we generated a basic universal protocol for the use of secondary antibodies selectively recognizing different isotypes/subclasses of monoclonal primary antibodies. This approach is widely applicable and offers a simple procedure for simultaneously detecting two or more antigens. (c) 2005 Elsevier GmbH. All rights reserved.
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