期刊
MICROBIOLOGICAL RESEARCH
卷 160, 期 4, 页码 385-388出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2005.03.002
关键词
fluorescent pseudomonad; mercury-resistant mutant; plant growth-promoting rhizobacteria (PGPR); enrichment
类别
Pseudomonas fluorescens strains PRS9 and GRS(1) (wild type) were made mercury resistant PRS9Hgr (147 mu M HgCl2) and GRS(1)Hg(r) (55 mu M HgCl2), respectively, in King's medium by enrichment selection and their in situ root colonization studies were carried out. Mercury resistant mutant of PRS9 was stable and resulted in significant increase in root and shoot fresh weight (P < 0.05). Both the mutants are positive for indoleacetic acid (IAA), 'P' solubilization and siderophore production. PRS9, potent 'P' solubilizer, exhibited higher 'P' solubilization as compared to GRS(1). After 2 weeks of inoculation, the population level of wild type PRS9 and its mercury resistant mutants has increased (50 fold). Mercury resistance has no adverse effect on the growth promoting properties of mutants besides being comparable in its morphological and physiological properties with their wild type counterpart. Furthermore, mercury resistant character facilitates rhizospheric competition and thus helpful for establishment of growth promoting strains where metal ions are either limiting and/or present at toxic level. (c) 2005 Elsevier GmbH. All rights reserved.
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