New complete structure of Hafnia alvei clinical isolate strain PCM 2670 semi-rough lipopolysaccharide

Hafnia alvei strain PCM 2670 is a clinical isolate from a patient with chronic reproductive tract infection. The novel structure of the semi-rough lipopolysaccharide was established with the use of NMR spectroscopy and mass spectrometry as well as immunochemical techniques. According to the mass spectrometry data, heptose in the oligosaccharide is partially substituted by glycine. H. alvei PCM 2670 core structure encompasses the common core of H. alvei which is modified with two additional galactose units. [GRAPHICS] The 6-substituted galactose is the O-antigen repeating unit substitution residue. The repeating unit consists of five monosaccharide residues and has the following structure: -> 2)-beta-Galp-(1 -> 6)-alpha-Glcp-( 1 -> 6)-alpha GlcpNAc3OAc-(1 -> 4)-alpha-GalpA-(1 -> 3)-beta-GlcpNAc6OAc-(1 -> 6)-core. (C) 2013 Elsevier Ltd. All rights reserved.