期刊
CARBOHYDRATE RESEARCH
卷 365, 期 -, 页码 38-45出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2012.10.005
关键词
Agarose; Gelation; Gradient NMR; Diffusion coefficient; Hydrodynamic screening length; Hydrodynamic mesh size
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [21550203]
- MECSST [22240076]
- Grants-in-Aid for Scientific Research [21550203, 22240076] Funding Source: KAKEN
Many biopolymers assume ordered structure in solution due to specific intermolecular interactions, and subsequently aggregate to form fibrous network structures, which play important structural and functional roles both in biomedical tissues and in biopolymeric applied materials. In this study, the pulsed-field-gradient stimulated echo (PGSTE) H-1 NMR method was utilized to elucidate the gelation mechanism and to determine the network structure of agarose. The echo signal intensity of agarose decreased with the formation of aggregated bundles, and therefore, it was used to determine the concentration of the solute agarose (c(sol)) in the gel. The diffusion coefficient of a dendrimer, added to the gel as a probe molecule, increased concomitantly with the formation of the network of aggregated bundles, suggesting apparent dilution of solute agarose in the network interspaces. The hydrodynamic mesh size (xi) of the network was estimated from the degree of retardation of the diffusion. The dependence of xi on csol was interpreted using a simple model, where the hydrodynamic interaction of the probe molecule with a solute chain or an aggregated bundle of chains is same. Our theoretically predicted lines fitted well on the experimentally obtained plots, thus validating the use of this model. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据