期刊
CARBOHYDRATE RESEARCH
卷 370, 期 -, 页码 13-18出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2013.01.016
关键词
Somatic hybrid mushroom (PfloVv5FB); Polysaccharide; Structure; NMR spectroscopy; Immunoactivation
资金
- DST, Govt. of India [SR/S1/OC-52/2006]
A water soluble polysaccharide isolated from the alkaline extract of the somatic hybrid mushroom (PfloVv5FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains was found to contain D-glucose only. Structural investigation was carried out using acid hydrolysis, methylation analysis; periodate oxidation, and NMR studies (H-1, C-13, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC). On the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide was established as: [GRAPHICS] This polysaccharide exhibited strong immunoactivation of macrophages, splenocytes as well as thymocytes. (C) 2013 Elsevier Ltd. All rights reserved.
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