Hepatoprotective glycosides from Leonurus japonicus Houtt

Two new phenylethanoid glycosides 1 and 2 named leonoside E and leonoside F, and one new sesquiterpene glycoside (3) identified as 7 alpha (H)-eudesmane-4,11 (12)-diene-3-one-2 beta-hydroxy-13-beta-D-glucopyranoside, together with seven known glycosides (4-10), were isolated from the aerial part of Leonurus japonicus Houtt. Their structures were elucidated on the basis of spectroscopic data and chemical evidence. When tested in in vitro assays, compounds 1, 2, 4, and 6 exhibited potent hepatoprotective activity against D-galactosamine-induced toxicity in HL-7702 cells at concentration of 1 x 10(-5) M. (C) 2011 Elsevier Ltd. All rights reserved.