期刊
CARBOHYDRATE RESEARCH
卷 346, 期 15, 页码 2451-2456出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2011.08.019
关键词
Hybrid mushroom; Polysaccharide; Structure; NMR spectroscopy
资金
- DST, Govt. of India [SR/S1/OC-52/2006]
- UGC, New Delhi
Three polysaccharide fractions (PS-I. PS-II, and PS-Ill) were isolated from the aqueous extract of a hybrid mushroom obtained through backcross mating of a somatic hybrid mushroom PfloVv12 (Sterile line) with Volvariella volvacea. PfloVv12 was obtained through protoplast fusion of Pleurotus florida and V. volvacea. PS-I was identified as 1,6-beta glucan. PS-II and PS-Ill were identified as mannoglucogalactan but differing in molecular weights only. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiment (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structures of these polysaccharides were established as; [GRAPHICS] (C) 2011 Elsevier Ltd. All rights reserved.
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