期刊
CARBOHYDRATE RESEARCH
卷 345, 期 9, 页码 1180-1189出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2010.03.042
关键词
Arabino-oligosaccharides; Sugar beet arabinan; Arabinohydrolases; NMR
资金
- Commission of the European Communities [MRTN-CT-2004-512265]
- Dutch Ministry of Economic Affairs
Sugar beet arabinan consists of an alpha-(1,5)-linked backbone of L-arabinosyl residues, which can be either single or double substituted with alpha-(1,2)- and/or alpha-(1,3)-linked L-arabinosyl residues. Neutral branched arabino-oligosaccharides were isolated from sugar beet arabinan by enzymatic degradation with mixtures of pure and well-defined arabinohydrolases from Chrysosporium lucknowense followed by fractionation based on size and analysis by MALDI-TOF MS and HPAEC. Using NMR analysis, two main series of branched arabino-oligosaccharides have been identified, both having an alpha-(1,5)-linked backbone of L-arabinosyl residues. One series carries single substituted alpha-(1,3)-linked L-arabinosyl residues at the backbone, whereas the other series consists of a double substituted alpha-(1,2,3,5)-linked arabinan structure within the molecule. The structures of eight such branched arabino-oligosaccharides were established. (C) 2010 Elsevier Ltd. All rights reserved.
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