期刊
CARBOHYDRATE RESEARCH
卷 345, 期 15, 页码 2124-2132出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2010.07.027
关键词
Cyclodextran; Periodate oxidation; Reductive amination; Lectin
资金
- Okinawa Prefecture, Japan
- Tropical Technology Center Ltd
The synthesis of small glycoclusters with high affinity toward lectins is one of the important subjects in glycotechnology. Although cyclic alpha-(1 -> 6)-D-octaglucoside (CI8) is an attractive scaffold on which to put glycosyl pendants, the compound has only secondary hydroxyl groups, which are relatively unreactive for substitution reactions. The oxidation of the vicinal diols of CI8 and reductive amination of the resultant dialdehydes with 2-aminoethyl mannoside gave mannose-CI8 conjugates with a variety of average mannose incorporation numbers (2-7). The average numbers were deduced from MALDI-TOF mass and H-1 NMR spectroscopy. The binding ability of mannose-CI8 conjugates to concanavalin A increased with the increasing numbers of average mannose incorporation, reaching a plateau at tetravalence, as estimated from a latex bead-based agglutination lectin assay. Toxicity tests demonstrated the biocompatibility of mannose-CI8 conjugates. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据