期刊
CARBOHYDRATE RESEARCH
卷 344, 期 13, 页码 1758-1763出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2009.06.008
关键词
Glycomimetics; Glycosyl disulfides; Isothermal titration calorimetry; Ligand-lectin interactions; Saturation transfer difference (STD) NMR
资金
- Hungarian Science Research Fund (OTKA) [T-48713, NK-68578]
- Department of Science and Technology, New Delhi
- Indo-Hungarian scientific exchange program [TeT IND-10/03, DST/INT/HUN/P-06/03]
alpha-D-Mannopyranosyl units were attached to an aromatic scaffold through disulfide linkages to obtain mono- to trivalent glycosylated ligands for lectin binding studies. Isothermal titration calorimetric (ITC) measurements indicated that binding affinities of these derivatives to Concanavalin A (Con A) were comparable to or slightly higher than that of methyl alpha-D-mannopyranoside (K-a values in the range of 10(4) M-1). The stoichiometries of the lectin-ligand complexes were in agreement with the formal valencies (1-3) of the respective ligands indicating cross-linking in interactions with the di- and trivalent derivatives. Multivalency effects could not, however, be observed with the latter. These ligands were shown to bind to the carbohydrate binding site of Con A using saturation transfer difference (STD) NMR competition experiments. (C) 2009 Elsevier Ltd. All rights reserved.
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