期刊
CARBOHYDRATE RESEARCH
卷 343, 期 14, 页码 2361-2368出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2008.07.004
关键词
sialyl Lewis(X) antigen; glycolipid; NBD; microdomain; fluorescence labeling; selectin
资金
- Deutsche Forschungsgemeinschaft
- Fonds der Chemischen Industrie
Selectin-induced leukocyte rolling along the endothelial surface is an essential step in the cellular immune response. For efficient recognition, the relevant carbohydrate epitope sialyl Lewis(X) (sLe(X); alpha-Neu-p5Ac-(2-->3)-beta-Galp-(1-->4)-[alpha-Fucp-(1-->3)]GlcpNAc) has to be arranged in clusters. We describe the synthesis of the sLe(X)-glycosphingolipid (sLe(X)-GSL) with a NBD fluorescence label in the tail region, which allows the direct visualization of sLe(X)-GSL microdomains to very low concentrations (0.01 mol %) in various planar phosphocholine matrices by fluorescence microscopy. Cell rolling experiments of E-selectin expressing cells along these membranes confirmed that the fluorescence analog behaves similar to the naturally occuring sLe(X)-GSL. This is direct evidence for recent hypotheses on multivalent sLe(X) binding as molecular basis for selectin-mediated cell rolling. (C) 2008 Elsevier Ltd. All rights reserved.
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