期刊
CARBOHYDRATE POLYMERS
卷 106, 期 -, 页码 270-275出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.02.040
关键词
Cordyceps sinensis; Mycelial fermentation; Exopolysaccharide; Ultrasonic degradation; (1 -> 3)-beta-D-glucan; Moisture absorption
资金
- Hong Kong Government UGC (GRF) [PolyU 5036/10P, PolyU 5033/11P]
- Hong Kong Polytechnic University
A high molecular weight (MW) exopolysaccaride (EPS) fraction EPS1 was isolated from the fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1 and partially degraded by high-intensity ultrasound (US) into a lower MW fraction EPS1U. EPS1U exhibited a single, symmetric peak on size exclusion chromatography with an average MW of 730 kDa by light scattering analysis. It had a much lower intrinsic viscosity (1.7 versus 15.6 dL/g) but a much higher solubility in water (77.5 versus 5.1 g/L) than EPS1U Based on methylation analysis and NMR spectrometry, the structure of EPS1U was deduced as a (1 -> 3)-beta-D-glucan with glucose side chains attached to 0-6 position at the branching points. EPS1U showed a high moisture absorption capability comparable to chitosan and urea, suggesting its potential as a moisturizing agent for food and cosmeceutical application. This is the first report on a high MW (1 -> 3)-beta-D-glucan isolated from EPS produced by Cordyceps sinensis. (C) 2014 Elsevier Ltd. All rights reserved.
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